全文获取类型
收费全文 | 367071篇 |
免费 | 41331篇 |
国内免费 | 165篇 |
出版年
2018年 | 3876篇 |
2017年 | 3571篇 |
2016年 | 5252篇 |
2015年 | 7248篇 |
2014年 | 8137篇 |
2013年 | 11320篇 |
2012年 | 12723篇 |
2011年 | 12631篇 |
2010年 | 8385篇 |
2009年 | 7613篇 |
2008年 | 10679篇 |
2007年 | 11099篇 |
2006年 | 10292篇 |
2005年 | 10288篇 |
2004年 | 10362篇 |
2003年 | 9702篇 |
2002年 | 9282篇 |
2001年 | 15519篇 |
2000年 | 15490篇 |
1999年 | 12439篇 |
1998年 | 4392篇 |
1997年 | 4435篇 |
1996年 | 4303篇 |
1995年 | 4203篇 |
1994年 | 4222篇 |
1993年 | 4072篇 |
1992年 | 10583篇 |
1991年 | 10085篇 |
1990年 | 9865篇 |
1989年 | 9594篇 |
1988年 | 8983篇 |
1987年 | 8554篇 |
1986年 | 7779篇 |
1985年 | 7860篇 |
1984年 | 6552篇 |
1983年 | 5695篇 |
1982年 | 4464篇 |
1981年 | 3986篇 |
1980年 | 3782篇 |
1979年 | 6475篇 |
1978年 | 4852篇 |
1977年 | 4545篇 |
1976年 | 4191篇 |
1975年 | 4576篇 |
1974年 | 4921篇 |
1973年 | 4984篇 |
1972年 | 4559篇 |
1971年 | 4239篇 |
1970年 | 3576篇 |
1969年 | 3500篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
91.
92.
Studies of the degradation of the two isomeric forms of butyrate in different anaerobic environments showed isomerization betweenn- andi-butyrate. Degradation rates were similar for the different examined systems and degradation rates forn-butyrate degradation were generally higher than fori-butyrate. Degradation rates forn-butyrate ranged from 0.52 to 1.39 day–1, while the rates fori-butyrate were from 0.46 to 1.15 day–1. Production of isomers was not observed when the volatile fatty acid degradation was inhibited by addition of bromoethane sulfonic acid, indicating that isomerization was coupled to the methanogenic degradation of the acid. The degree of isomerization observed duringn-butyrate degradation was similar to the degree duringi-butyrate degradation. Experiments indicated that the isomerization degree was higher for the thermophilic than for the mesophilic inocula. 相似文献
93.
94.
A novel 145 kd brain cytosolic protein reconstitutes Ca(2+)-regulated secretion in permeable neuroendocrine cells. 总被引:17,自引:0,他引:17
The regulated secretory pathway is activated by elevated cytoplasmic Ca2+; however, the components mediating Ca2+ regulation have not been identified. In semi-intact neuroendocrine cells, Ca(2+)-activated secretion is ATP- and cytosol protein-dependent. We have identified a novel brain protein, p145, as a cytosolic factor that reconstitutes Ca(2+)-activated secretion in two neuroendocrine cell types. The protein is a dimer of 145 kd subunits, exhibits Ca(2+)-dependent interaction with a hydrophobic matrix, and binds phospholipid vesicles, suggesting a membrane-associated function. A p145-specific antibody inhibits the reconstitution of Ca(2+)-activated secretion by cytosol, indicating an essential role for p145. The restricted expression of p145 in tissues exhibiting a regulated secretory pathway suggests a key role for this protein in the transduction of Ca2+ signals into vectorial membrane fusion events. 相似文献
95.
G J van Ingen Schenau F C Bakker G de Groot J J de Koning 《European journal of applied physiology and occupational physiology》1992,64(4):292-297
Seven female and eight male elite junior skaters performed cycle ergometer tests at four different times during the 1987/1988 season. The tests consisted of a Wingate-type 30-s sprint test and a 2.5-min supramaximal test. The subjects were tested in February, May and September 1987 and in January 1988. Maximal oxygen consumption was measured during the 2.5-min test. With the exception of the maximal oxygen consumption of the women in May which was about 6% lower than in the other three tests, no seasonal changes in the test results could be observed--this, in spite of a distinct increase in training volume (from 10 to more than 20 h.week-1) and training intensity in the course of the season. When the test data were compared to those of elite senior skaters, it appeared that the junior skaters showed the same values for mean power output during the sprint test [14.2 (SD 0.4) W.kg-1 for the men and 12.6 (SD 0.5) W.kg-1 for the women] and maximal oxygen consumption [63.1 (SD 2.8) ml.kg-1.min-1 for the men and 55.3 (SD 3.5) ml.kg-1.min-1 for the women, respectively] as found for senior skaters. It seemed, therefore, that the effects of training in these skaters had already levelled off in the period before they participated in this investigation. In contrast to previous studies, no relationship could be shown between the test results and skating performance. This was most likely due to the homogenous character of the groups (mean standard deviations in power and oxygen consumption were only 5%).(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
96.
97.
A mutation in apolipoprotein A-I in the Iowa type of familial amyloidotic polyneuropathy 总被引:15,自引:0,他引:15
Immunoblotting of isoelectric focusing gels of plasma and direct genomic DNA sequencing have been used to characterize a mutation in apolipoprotein A-I associated with the familial amyloidotic polyneuropathy originally described by Van Allen in an Iowa kindred. An arginine for glycine substitution in apolipoprotein A-I identified in the proband's amyloid fibrils was determined to be the result of a mutation of guanine to cytosine in the apolipoprotein A-I gene at the position corresponding to the first base of codon 26. Direct sequencing of genomic DNA of three affected individuals who died in the 1960s confirmed the inheritance of the disorder. Immunoblot analysis detected the variant apolipoprotein A-I in the proband's plasma and in several at-risk members of the kindred. In addition, allele-specific amplification by the polymerase chain reaction was used to detect carriers of the variant gene. 相似文献
98.
Aaron B. Bernstein Elisabeth Preisig Hubert E. Schroeder 《Cell and tissue research》1990,259(3):603-606
Summary A diseased and mechanically treated surface of root cementum is known, clinically, to favor periodontal regeneration. The present investigation was undertaken to test whether previously diseased and experimentally treated root surfaces can support the in-vitro formation of a new collagenous matrix. Three teeth extracted for advanced periodontitis were treated first with 5% sodium hypochlorite for 2 h to remove all organic material from the root surface. After the healthy, apical one third of the root was cut off, the roots were scaled with moderate pressure to remove visible calculus. Non-demineralized root discs were cut and placed on a co-culture of periodontal ligament- and alveolar bone-derived cells. After 7 weeks in culture, either one of two matrix types was found along the root surface. The most frequent matrix consisted of clusters of cells layered within densely aggregated collagen fibrils. The other, less frequent matrix consisted of loosely arranged collagen fibrils adjacent to the cemental surface. The findings support the notion that, in vitro, a collagenous matrix is formed in contact to diseased and experimentally treated root surfaces. However, the smooth, non-demineralized and scaled cemental surface does not appear to be a suitable substrate for interdigitation with newly produced collagen fibrils. 相似文献
99.
The effect of hydrogen peroxide on ADP-induced platelet aggregation in the presence of active oxygen species scavengers was studied. It was shown that the superoxide radical and singlet oxygen, alongside with hydrogen peroxide, may play a role in platelet interactions. 相似文献
100.
E Bignon A Kishimoto M Pons A Crastes de Paulet J Gilbert J F Miquel Y Nishizuka 《Biochemical and biophysical research communications》1990,166(3):1471-1478
Protein kinase C (PKC) I (gamma), II (beta) and III (alpha) subspecies are all activated by 1,1-di-(p-hydroxyphenyl)ethylene derivatives (DPE) at micromolar concentrations. This PKC activation depends on the presence of both Ca2+ and phosphatidylserine (PS) but does not require diacylglycerol (DG). DPEs enhance PKC activity at low PS concentrations, but not at saturating PS concentrations. Like DG, DPEs increase the apparent affinity of PKC for PS as well as for Ca2+, but lead to a decrease in the catalytic activity (Vmax). In the presence of saturating DG concentrations, DPEs exhibit an inhibitory action. The derivatives also inhibit the activity of the proteolytic fragment of PKC, protein kinase M. It is concluded that DPEs are mixed-type inhibitors, probably interacting with the catalytic domain of the enzyme. 相似文献